Kidney micro-organoids in suspension culture as a scalable source of human pluripotent stem cell-derived kidney cells

nephron
stem cell
suspension culture
single-cell
rna-seq
kidney
organoids
Authors

Santhosh V Kumar

Pei X Er

Kynan T Lawlor

Ali Motazedian

Michelle Scurr

Irene Ghobrial

Alexander N Combes

Luke Zappia

Alicia Oshlack

Edouard G Stanley

Melissa H Little

Date

March 1, 2019

Links
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Abstract

Kidney organoids have potential uses in disease modelling, drug screening and regenerative medicine. However, novel cost-effective techniques are needed to enable scaled-up production of kidney cell types in vitro. We describe here a modified suspension culture method for the generation of kidney micro-organoids from human pluripotent stem cells. Optimisation of differentiation conditions allowed the formation of micro-organoids, each containing six to ten nephrons that were surrounded by endothelial and stromal populations. Single cell transcriptional profiling confirmed the presence and transcriptional equivalence of all anticipated renal cell types consistent with a previous organoid culture method. This suspension culture micro-organoid methodology resulted in a three- to fourfold increase in final cell yield compared with static culture, thereby representing an economical approach to the production of kidney cells for various biological applications.

Citation

BibTeX citation:
@article{v_kumar2019,
  author = {V Kumar, Santhosh and X Er, Pei and T Lawlor, Kynan and
    Motazedian, Ali and Scurr, Michelle and Ghobrial, Irene and N
    Combes, Alexander and Zappia, Luke and Oshlack, Alicia and G
    Stanley, Edouard and H Little, Melissa},
  title = {Kidney Micro-Organoids in Suspension Culture as a Scalable
    Source of Human Pluripotent Stem Cell-Derived Kidney Cells},
  journal = {Development},
  volume = {146},
  number = {5},
  date = {2019-03-01},
  url = {https://lazappi.id.au/publications/2019-kumar-micro-organoids/},
  doi = {10.1242/dev.172361},
  issn = {0950-1991, 1477-9129},
  langid = {en},
  abstract = {Kidney organoids have potential uses in disease modelling,
    drug screening and regenerative medicine. However, novel
    cost-effective techniques are needed to enable scaled-up production
    of kidney cell types in vitro. We describe here a modified
    suspension culture method for the generation of kidney
    micro-organoids from human pluripotent stem cells. Optimisation of
    differentiation conditions allowed the formation of micro-organoids,
    each containing six to ten nephrons that were surrounded by
    endothelial and stromal populations. Single cell transcriptional
    profiling confirmed the presence and transcriptional equivalence of
    all anticipated renal cell types consistent with a previous organoid
    culture method. This suspension culture micro-organoid methodology
    resulted in a three- to fourfold increase in final cell yield
    compared with static culture, thereby representing an economical
    approach to the production of kidney cells for various biological
    applications.}
}
For attribution, please cite this work as:
V Kumar, Santhosh, Pei X Er, Kynan T Lawlor, Ali Motazedian, Michelle Scurr, Irene Ghobrial, Alexander N Combes, et al. 2019. “Kidney Micro-Organoids in Suspension Culture as a Scalable Source of Human Pluripotent Stem Cell-Derived Kidney Cells.” Development 146 (March). https://doi.org/10.1242/dev.172361.