Kidney micro-organoids in suspension culture as a scalable source of human pluripotent stem cell-derived kidney cells
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Kidney organoids have potential uses in disease modelling, drug screening and regenerative medicine. However, novel cost-effective techniques are needed to enable scaled-up production of kidney cell types in vitro. We describe here a modified suspension culture method for the generation of kidney micro-organoids from human pluripotent stem cells. Optimisation of differentiation conditions allowed the formation of micro-organoids, each containing six to ten nephrons that were surrounded by endothelial and stromal populations. Single cell transcriptional profiling confirmed the presence and transcriptional equivalence of all anticipated renal cell types consistent with a previous organoid culture method. This suspension culture micro-organoid methodology resulted in a three- to fourfold increase in final cell yield compared with static culture, thereby representing an economical approach to the production of kidney cells for various biological applications.
Citation
@article{v_kumar2019,
author = {V Kumar, Santhosh and X Er, Pei and T Lawlor, Kynan and
Motazedian, Ali and Scurr, Michelle and Ghobrial, Irene and N
Combes, Alexander and Zappia, Luke and Oshlack, Alicia and G
Stanley, Edouard and H Little, Melissa},
title = {Kidney Micro-Organoids in Suspension Culture as a Scalable
Source of Human Pluripotent Stem Cell-Derived Kidney Cells},
journal = {Development},
volume = {146},
number = {5},
date = {2019-03-01},
url = {https://doi.org/10.1242/dev.172361},
doi = {10.1242/dev.172361},
issn = {0950-1991, 1477-9129},
langid = {en},
abstract = {Kidney organoids have potential uses in disease modelling,
drug screening and regenerative medicine. However, novel
cost-effective techniques are needed to enable scaled-up production
of kidney cell types in vitro. We describe here a modified
suspension culture method for the generation of kidney
micro-organoids from human pluripotent stem cells. Optimisation of
differentiation conditions allowed the formation of micro-organoids,
each containing six to ten nephrons that were surrounded by
endothelial and stromal populations. Single cell transcriptional
profiling confirmed the presence and transcriptional equivalence of
all anticipated renal cell types consistent with a previous organoid
culture method. This suspension culture micro-organoid methodology
resulted in a three- to fourfold increase in final cell yield
compared with static culture, thereby representing an economical
approach to the production of kidney cells for various biological
applications.}
}